Identification and mapping of random amplified polymorphic DNA markers linked to a rhizomania resistance gene in sugar beet (Beta vulgaris L.) by bulked segregant analysis
Identifieur interne : 003F64 ( Main/Exploration ); précédent : 003F63; suivant : 003F65Identification and mapping of random amplified polymorphic DNA markers linked to a rhizomania resistance gene in sugar beet (Beta vulgaris L.) by bulked segregant analysis
Auteurs : F. Pelsy [France] ; D. Merdinoglu [France]Source :
- Plant Breeding [ 0179-9541 ] ; 1996-11.
English descriptors
- Teeft :
- Allele, Appl, Arbitrary ohgonucleotide primers, Beet, Beet root, Beta, Beta vulgaris, Bnyvv, Bruxelles, Different sources, Disease phenotype, Dominant allele, Experimental protocol, Gene, Genetic component, Genetic variation, Genotype, Genotypic screening, Hnkage groups, Holly, Holly resistance, Holly resistance gene, Holly source, Holly sugar company, Iirb, Initial screening, Inra colmar, Interval mapping, Lewellen, Linkage, Linkage analysis, Linkage group, Linkage groups, Linkage maps, Linolenic acid concentration, Major genes, Marker, Merdinoglu, Negative control, Ofthe, Optical density, Optical density value, Partial dominance, Polymorphic, Polymorphic bands, Polymorphism, Polymyxa betae, Primer, Rapd, Rapd markers, Resistance allele, Resistance gene, Resistance genes, Resistant, Resistant allele, Resistant bulk, Resistant cultivars, Resistant individuals, Resistant parent, Rhizomania, Rhizomania resistance, Rhizomania resistance gene, Rhizomania resistances, Segregant, Segregant analysis, Segregation analysis, Selection ofthe, Significant effect, Significant markers, Single gene, Sugar beet, Sugar beet genome, Susceptible, Susceptible allele, Susceptible bulk, Susceptible individuals, Susceptible parent, Susceptible parents, Susceptible plants, Target gene, Threshold value, Total variation, Vulgaris, Winter cong, Yellow vein virus.
Abstract
Bulked segregant analysis was employed to identify random amplified polymorphic DNA (RAPD) markers linked to a gene that confers rhizomania resistance to a sugar beet line created from a Holly Sugar Company breeding population (USA). Polymorphism revealed with 160 arbitrary 10‐mer oligonucleotide primers was screened in two bulks produced by separately pooling the individual DNAs from the six most resistant and the six most susceptible plants of an F2 population segregating for rhizomania resistance. A study of the F2 individuals showed that 19 primers generated 44 polymorphic markers which were then grouped into nine linkage groups. By analysis of variance, 12 were shown to have a significant effect upon the level of resistance and were mapped on a segment 22.3 cM long. A quantitative trait locus (QTL) of resistance was identified and located in a 4.6cM interval between two markers. It accounted for 67.4% of the observed variation and almost all the genetic variation. These results suggest that the identified QTL corresponds to a unique major gene conditioning the Holly resistance studied, which we have named Rz‐l.
Url:
DOI: 10.1111/j.1439-0523.1996.tb00936.x
Affiliations:
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Merdinoglu</name><affiliation wicri:level="3"><country xml:lang="fr">France</country><wicri:regionArea>INRA, Station des Grandes Cultures, Laboratoire de Pathologic Vegetale, F‐68021 Colmar Cedex</wicri:regionArea><placeName><region type="region" nuts="2">Grand Est</region><region type="old region" nuts="2">Alsace (région administrative)</region><settlement type="city">Colmar</settlement></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j" type="main">Plant Breeding</title><title level="j" type="alt">PLANT BREEDING</title><idno type="ISSN">0179-9541</idno><idno type="eISSN">1439-0523</idno><imprint><biblScope unit="vol">115</biblScope><biblScope unit="issue">5</biblScope><biblScope unit="page" from="371">371</biblScope><biblScope unit="page" to="377">377</biblScope><biblScope unit="page-count">7</biblScope><publisher>Blackwell Publishing Ltd</publisher><pubPlace>Oxford, UK</pubPlace><date type="published" when="1996-11">1996-11</date></imprint><idno type="ISSN">0179-9541</idno></series></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0179-9541</idno></seriesStmt></fileDesc><profileDesc><textClass><keywords scheme="Teeft" xml:lang="en"><term>Allele</term><term>Appl</term><term>Arbitrary ohgonucleotide primers</term><term>Beet</term><term>Beet root</term><term>Beta</term><term>Beta vulgaris</term><term>Bnyvv</term><term>Bruxelles</term><term>Different sources</term><term>Disease phenotype</term><term>Dominant allele</term><term>Experimental protocol</term><term>Gene</term><term>Genetic component</term><term>Genetic variation</term><term>Genotype</term><term>Genotypic screening</term><term>Hnkage groups</term><term>Holly</term><term>Holly resistance</term><term>Holly resistance gene</term><term>Holly source</term><term>Holly sugar company</term><term>Iirb</term><term>Initial screening</term><term>Inra colmar</term><term>Interval mapping</term><term>Lewellen</term><term>Linkage</term><term>Linkage analysis</term><term>Linkage group</term><term>Linkage groups</term><term>Linkage maps</term><term>Linolenic acid concentration</term><term>Major genes</term><term>Marker</term><term>Merdinoglu</term><term>Negative control</term><term>Ofthe</term><term>Optical density</term><term>Optical density value</term><term>Partial dominance</term><term>Polymorphic</term><term>Polymorphic bands</term><term>Polymorphism</term><term>Polymyxa betae</term><term>Primer</term><term>Rapd</term><term>Rapd markers</term><term>Resistance allele</term><term>Resistance gene</term><term>Resistance genes</term><term>Resistant</term><term>Resistant allele</term><term>Resistant bulk</term><term>Resistant cultivars</term><term>Resistant individuals</term><term>Resistant parent</term><term>Rhizomania</term><term>Rhizomania resistance</term><term>Rhizomania resistance gene</term><term>Rhizomania resistances</term><term>Segregant</term><term>Segregant analysis</term><term>Segregation analysis</term><term>Selection ofthe</term><term>Significant effect</term><term>Significant markers</term><term>Single gene</term><term>Sugar beet</term><term>Sugar beet genome</term><term>Susceptible</term><term>Susceptible allele</term><term>Susceptible bulk</term><term>Susceptible individuals</term><term>Susceptible parent</term><term>Susceptible parents</term><term>Susceptible plants</term><term>Target gene</term><term>Threshold value</term><term>Total variation</term><term>Vulgaris</term><term>Winter cong</term><term>Yellow vein virus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Bulked segregant analysis was employed to identify random amplified polymorphic DNA (RAPD) markers linked to a gene that confers rhizomania resistance to a sugar beet line created from a Holly Sugar Company breeding population (USA). Polymorphism revealed with 160 arbitrary 10‐mer oligonucleotide primers was screened in two bulks produced by separately pooling the individual DNAs from the six most resistant and the six most susceptible plants of an F2 population segregating for rhizomania resistance. A study of the F2 individuals showed that 19 primers generated 44 polymorphic markers which were then grouped into nine linkage groups. By analysis of variance, 12 were shown to have a significant effect upon the level of resistance and were mapped on a segment 22.3 cM long. A quantitative trait locus (QTL) of resistance was identified and located in a 4.6cM interval between two markers. It accounted for 67.4% of the observed variation and almost all the genetic variation. These results suggest that the identified QTL corresponds to a unique major gene conditioning the Holly resistance studied, which we have named Rz‐l.</div></front></TEI><affiliations><list><country><li>France</li></country><region><li>Alsace (région administrative)</li><li>Grand Est</li></region><settlement><li>Colmar</li></settlement></list><tree><country name="France"><region name="Grand Est"><name sortKey="Pelsy, F" sort="Pelsy, F" uniqKey="Pelsy F" first="F." last="Pelsy">F. Pelsy</name></region><name sortKey="Merdinoglu, D" sort="Merdinoglu, D" uniqKey="Merdinoglu D" first="D." last="Merdinoglu">D. Merdinoglu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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